circRNA basic information
circBase ID: hsa_circ_0014235
Name: hsa_circ_S100A2
Synonym: circ_0014235
Host Gene: S100A2
Genomic location(hg19): chr1:153536209-153536363:-
Genomic location(hg38): chr1:153563733-153563887:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues

Cell lines:

PC9; HCC827; PC9/GR; HCC827/GR; 293 T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: YAP, PD-L1, S100A2
Associated microRNA: miR-146b-5p
Biological function: Confers Gefitinib resistance and malignant behaviors; promotes Gefitinib IC50, cell proliferation, colony formation, tumor growth and immune escape; inhibits apoptosis and cell cycle arrest in Gefitinib-resistant NSCLC.
Molecular mechanism: circ_0014235 acts through a ceRNA-like mechanism by targeting miR-146b-5p, relieving miR-146b-5p-mediated inhibition of YAP, and subsequently regulating PD-L1 expression.
Biological pathway or process:

drug resistance (promotes); chemoresistance (promotes); proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); immune regulation (promotes); Hippo/YAP (promotes); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis

Clinical significance:

-

Description:

circ_0014235 is up-regulated in Gefitinib-resistant NSCLC cells and promotes Gefitinib resistance, proliferation, colony formation, tumor growth and immune escape while suppressing apoptosis and cell cycle arrest. Mechanistically, circ_0014235 targets miR-146b-5p to relieve inhibition of YAP and regulate PD-L1 expression, forming a circ_0014235/miR-146b-5p/YAP/PD-L1 pathway.

Confidence score:

0.795

Other information
Title:

Circ_0014235 confers Gefitinib resistance and malignant behaviors in non-small cell lung cancer resistant to Gefitinib by governing the miR-146b-5p/YAP/PD-L1 pathway.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2022
PubMed ID: 34919024
Study type:

biological research

Data availability: -
Code availability: -