circRNA basic information
circBase ID: hsa_circ_0002360
Name: hsa_circ_RUNX1
Synonym: -
Host Gene: RUNX1
Genomic location(hg19): chr21:36206706-36231875:-
Genomic location(hg38): chr21:34834409-34859578:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: Non-small cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent normal tissue samples; tumor tissues
Cell lines:

H522; A549; H1299; H460; BEAS-2B; LLC
In vivo animal model:

cell line-derived xenograft; syngeneic model
circRNA-disease information
Expression pattern:
UP
Associated gene: HNRNPA1, PD-L1, TGFB1, p-SMAD2, CD8 + T cells
Associated microRNA: -
Biological function: Promotes NSCLC cell proliferation, invasion, migration, EMT, tumor growth and immune evasion; inhibits apoptosis and CD8 + T cell cytotoxicity.
Molecular mechanism: hsa_circ_0002360 directly binds HNRNPA1, enhances HNRNPA1 mRNA stability, increases HNRNPA1 levels, and promotes PD-L1 expression and TGF-beta/SMAD signaling to facilitate malignant progression and immune evasion.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits); TGF-beta/SMAD (promotes); mRNA stability (promotes); immune regulation (promotes)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; Transwell Assay; In Vivo Animal Model; IF (Immunofluorescence); Western Blot; Bioinformatics Analysis
Clinical significance:

hsa_circ_0002360 overexpression was significantly associated with advanced TNM stages and distant metastasis in NSCLC, suggesting potential therapeutic value.
Description:

hsa_circ_0002360 is up-regulated in NSCLC tissues and cell lines and is associated with advanced TNM stage and distant metastasis. Functionally, it promotes proliferation, invasion, migration, EMT, tumor growth and immune evasion while suppressing apoptosis. Mechanistically, it interacts with HNRNPA1, enhances HNRNPA1 mRNA stability, and activates PD-L1/TGF-beta-related immune evasion programs.
Confidence score:

0.8355
Other information
Title:

Has_circ_0002360 facilitates immune evasion by enhancing heterogeneous nuclear ribonucleoprotein A1 stability, thereby promoting malignant progression in non-small cell lung cancer.

Journal: Experimental cell research
Published: 2024
PubMed ID: 39476941
Study type:

combined biological and clinical study
Data availability: GSE112214; GSE158695; GPL19978; Supplementary data; https://doi.org/10.1016/j.yexcr.2024.114312
Code availability: -