| Expression pattern: |
UP |
| Associated gene: |
PD-L1, CD8+ T cells, IFN-gamma |
| Associated microRNA: |
miR-4477a |
| Biological function: |
circFOXP1 acts as an oncogenic regulator in GBC by increasing PD-L1 expression, suppressing CD8+ T cell function, promoting GBC cell viability, and inhibiting apoptosis and ferroptosis; Rg3 downregulates circFOXP1 to restore CD8+ T cell activity and induce ferroptosis and apoptosis. |
| Molecular mechanism: |
ceRNA mechanism: circFOXP1 sponges miR-4477a to upregulate PD-L1, thereby inhibiting CD8+ T cell function; Rg3 suppresses circFOXP1, releases miR-4477a-mediated downregulation of PD-L1, activates CD8+ T cells, and promotes ferroptosis and apoptosis. |
| Biological pathway or process: |
ceRNA regulation (other); immune regulation (inhibits); ferroptosis (inhibits); apoptosis (inhibits); proliferation (promotes); drug resistance (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Flow Cytometry（Non-apoptosis/cycle）; ELISA; IF (Immunofluorescence); Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); TUNEL |
| Clinical significance: |
circFOXP1 was significantly upregulated in GBC tumor tissues and may be a potential therapeutic target in the Rg3-mediated anti-cancer mechanism. |
| Description: |
circFOXP1 is upregulated in GBC tumor tissues and functions as a miR-4477a sponge to increase PD-L1 expression. Through this circFOXP1-miR-4477a-PD-L1 axis, it suppresses CD8+ T cell activity and limits ferroptosis and apoptosis in GBC cells; Rg3 counteracts this axis by downregulating circFOXP1. |
| Confidence score: |
0.7554 |