circRNA basic information
circBase ID: hsa_circ_0046854
Name: hsa_circ_ANKRD12
Synonym: -
Host Gene: ANKRD12
Genomic location(hg19): chr18:9263787-9275665:+
Genomic location(hg38): chr18:9263789-9275667:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; normal tissues; paracancerous tissue samples; HP-negative GC tissues; HP-positive GC tissues; xenograft tumor tissues; lung tissues; blood samples
Cell lines:

GES-1; BGC-823; BGC-823/DDP
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CSF1, CagA, ANKRD12
Associated microRNA: miR-511-3p
Biological function: Promotes gastric cancer cell growth, proliferation, migration, invasion, anti-apoptosis, tumor growth, and cisplatin/DDP resistance; knockdown reverses CagA+ Helicobacter pylori-induced malignant phenotypes and enhances the inhibitory effects of DDP in vivo.
Molecular mechanism: Helicobacter pylori induces circ_0046854 expression in a CagA-dependent manner; circ_0046854 functions as a miR-511-3p sponge to regulate CSF1, thereby mediating gastric cancer progression and DDP resistance.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); chemoresistance (promotes); drug resistance (promotes)
Detected method:
Q
S
Validation methods:

RT-qPCR; RNA-seq; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis
Clinical significance:

circ_0046854 is highly expressed in GC tissues, especially HP-positive GC tissues, and the circ_0046854/miR-511-3p/CSF1 axis may serve as a potential therapeutic target for HP-associated GC.
Description:

In this study, circ_0046854 is a human ANKRD12-derived circRNA up-regulated in gastric cancer, especially HP-positive gastric cancer. Helicobacter pylori induces circ_0046854 through CagA, and circ_0046854 promotes GC cell growth, invasion, migration, anti-apoptosis, tumor growth, and cisplatin/DDP resistance by sponging miR-511-3p and regulating CSF1. Silencing circ_0046854 suppresses tumor growth and enhances the anti-tumor effect of DDP, suggesting the circ_0046854/miR-511-3p/CSF1 axis as a potential therapeutic target for HP-associated GC.
Confidence score:

0.8571
Other information
Title:

Helicobacter pylori induce circ_0046854 to regulate microRNA-511-3p/CSF1 axis and enhance the resistance of gastric cancer to cisplatin.

Journal: Journal of biochemical and molecular toxicology
Published: 2024
PubMed ID: 39291620
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request
Code availability: -