| Expression pattern: |
UP |
| Associated gene: |
IL-1beta, AGO2 |
| Associated microRNA: |
miR-4731-5p |
| Biological function: |
facilitates endometriosis progression, recruits immune cell infiltration, promotes CD4+ T cell, macrophage and neutrophil infiltration, and increases IL-1beta synthesis and secretion |
| Molecular mechanism: |
circGLIS2 acts as a ceRNA/molecular sponge for miR-4731-5p to regulate IL-1beta expression, leading to immune cell infiltration in the endometriosis microenvironment |
| Biological pathway or process: |
ceRNA regulation (promotes); immune regulation (promotes); inflammation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; IHC (Immunohistochemistry); IF (Immunofluorescence); ELISA; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Bioinformatics Analysis |
| Clinical significance: |
circGLIS2 expression was related to clinicopathologic characteristics, rASRM staging and scoring, and dysmenorrhoea symptoms in EMs patients |
| Description: |
Human circGLIS2 is up-regulated in endometriosis tissues and correlates with clinicopathologic features including rASRM staging/scoring and dysmenorrhoea. It predominantly localizes in the cytoplasm and promotes immune cell infiltration by sponging miR-4731-5p to increase IL-1beta expression and secretion. |
| Confidence score: |
0.8748 |