| Expression pattern: |
UP |
| Associated gene: |
ACLY, CTNNB1, CCND1, YTHDF1 |
| Associated microRNA: |
- |
| Biological function: |
Promotes CRC cell proliferation, colony formation, migration, invasion, tumor growth and lung metastasis; affects cell cycle by causing G1 phase arrest when silenced. |
| Molecular mechanism: |
CircAURKA inhibits CTNNB1 protein degradation by promoting the interaction between ACLY and CTNNB1; it reduces CTNNB1 ubiquitination and acts in a CTNNB1-dependent manner. CircAURKA stability is regulated by m6A methylation modification and YTHDF1 enrichment. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); cell cycle (promotes); ubiquitination (inhibits); m6A modification (other) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; ISH (In Situ Hybridization); IHC (Immunohistochemistry); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; MeRIP / MeRIP-seq; Transfection; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; Western Blot; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
CircAURKA has potential diagnostic value in serum for CRC patients; high expression is not favorable to prognosis and is associated with lymph node metastasis. |
| Description: |
CircAURKA (hsa_circ_0008777), derived from AURKA, is up-regulated in CRC tissues, cells and serum and is associated with poorer prognosis, lymph node metastasis and potential diagnostic value. Functionally, it promotes CRC proliferation, invasion and metastasis in vitro and in vivo. Mechanistically, circAURKA promotes ACLY-CTNNB1 interaction, inhibits CTNNB1 ubiquitination/degradation, supports Wnt/beta-catenin-related downstream effects, and its own stability is regulated by m6A modification. |
| Confidence score: |
0.9026 |