| Expression pattern: |
UP |
| Associated gene: |
PRMT5, PD-L1, H3K4me3 |
| Associated microRNA: |
miR-338-3p |
| Biological function: |
promotes cell proliferation, migration, invasion, immune evasion, and tumor progression |
| Molecular mechanism: |
Acts as a ceRNA sponge for miR-338-3p to upregulate PRMT5, which enhances H3K4me3 recruitment at the PD-L1 promoter and increases PD-L1 transcription/expression, promoting immune evasion. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); immune regulation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Sanger Sequencing; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; Transwell Assay; Wound Healing Assay; Colony Formation Assay; Western Blot; ChIP / ChIP-seq; Annexin V/PI Flow Cytometry; ELISA; Survival Analysis |
| Clinical significance: |
potential diagnostic and prognostic marker for BC (not clinically validated in this study) |
| Description: |
circGSK3beta is upregulated in breast cancer cell lines and promotes proliferation, migration, and invasion. It acts as a ceRNA by sponging miR-338-3p to increase PRMT5, which enhances H3K4me3 at the PD-L1 promoter and upregulates PD-L1, leading to immune evasion via impaired CD8+ T-cell function. |
| Confidence score: |
0.8278 |