| Expression pattern: |
UP |
| Associated gene: |
Raf1, MEK1, ERKs, p-MEK1, p-ERK |
| Associated microRNA: |
miR-1287 |
| Biological function: |
promotes HCC cell proliferation, migration, invasion, EMT, and tumor growth |
| Molecular mechanism: |
acts as a miR-1287 sponge to upregulate Raf1 and activate the MEK/ERK pathway |
| Biological pathway or process: |
MEK/ERK (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; Colony Formation Assay; MTT; Transwell Assay; Western Blot; Clinical Sample Validation; In Vivo Animal Model |
| Clinical significance: |
circ_CDR1as might be a diagnostic biomarker for HCC patients |
| Description: |
circ_CDR1as is up-regulated in HCC tissues and cell lines and promotes proliferation, migration/invasion, EMT and xenograft tumor growth. Mechanistically, it sponges miR-1287 to increase Raf1 expression and thereby activates the MEK/ERK pathway. |
| Confidence score: |
0.7631 |