| Expression pattern: |
UP |
| Associated gene: |
STAT3, TNF-𝛼, IL-6, CD11b, CD45, iNOS, Iba-1, GFAP, Arg-1 |
| Associated microRNA: |
- |
| Biological function: |
circATF7IP is associated with MDD pathogenesis; its downregulation by SALNP3-si-circATF7IP alleviates LPS-induced depressive-like behaviors, suppresses microglial activation, reduces pro-inflammatory cytokine production, and alleviates neuroinflammation. |
| Molecular mechanism: |
Downregulation of circATF7IP inhibits STAT3 nuclear translocation in LPS-stimulated microglia and reduces pro-inflammatory immune responses, including TNF-𝛼 and IL-6 production and microglial activation. |
| Biological pathway or process: |
inflammation (promotes); immune regulation (promotes); JAK/STAT (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; Transfection; CCK8; ELISA; Western Blot; IF (Immunofluorescence); Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
circATF7IP was positively correlated with HAMD-24 scores, could differentiate MDD patients from healthy controls with high sensitivity and specificity, and its level decreased in patients with good treatment outcomes. |
| Description: |
circATF7IP is upregulated in plasma from MDD patients and in depressed mouse models, and its level correlates with MDD severity and treatment outcome. Intranasal SALNP delivery of si-circATF7IP downregulates circATF7IP and alleviates LPS-induced depressive-like behaviors by reducing neuroinflammation, microglial activation, STAT3 nuclear translocation, and TNF-𝛼/IL-6 production. |
| Confidence score: |
0.678 |