| Expression pattern: |
UP |
| Associated gene: |
circFOXP1-231aa, OTUD4, NCOA4 |
| Associated microRNA: |
- |
| Biological function: |
inhibits ICC progression, proliferation, colony formation, invasion, tumor growth and recurrence; enhances ferroptosis |
| Molecular mechanism: |
circFOXP1 encodes circFOXP1-231aa; circFOXP1-231aa binds OTUD4; OTUD4 suppresses ubiquitination of NCOA4, stabilizes NCOA4 protein, and promotes ferroptosis |
| Biological pathway or process: |
ferroptosis (promotes); proliferation (inhibits); invasion (inhibits); ubiquitination (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; RT-qPCR; FISH / smFISH; ISH (In Situ Hybridization); IHC (Immunohistochemistry); Clinical Sample Validation; Co-IP; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
high circFOXP1 expression is associated with longer OS, lower recurrence rates, better prognosis, and may serve as a prognostic factor for patients with ICC |
| Description: |
circFOXP1 is highly expressed in ICC tumors from patients without postoperative recurrence and high expression predicts better prognosis. It suppresses ICC proliferation, invasion, tumor growth, and recurrence by encoding circFOXP1-231aa, which binds OTUD4, inhibits NCOA4 ubiquitination/degradation, and enhances ferroptosis. |
| Confidence score: |
0.8814 |