| Expression pattern: |
UP |
| Associated gene: |
GFRA1, WTAP |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
promotes OS cell proliferation, migration, invasion, angiogenesis, and tumor growth; inhibits apoptosis |
| Molecular mechanism: |
WTAP-mediated m6A modification facilitates circ_0032463 biogenesis; circ_0032463 acts as a ceRNA sponge for miR-145-5p to increase GFRA1 expression |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); angiogenesis (promotes); metastasis (promotes); ceRNA regulation (other); m6A modification (other); mRNA stability (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); Transfection; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
suggesting a promising molecular marker for OS treatment; provide a promising target for OS therapy |
| Description: |
circ_0032463 is up-regulated in osteosarcoma tissues and cells and mainly localizes in the cytoplasm. It promotes malignant OS phenotypes including proliferation, migration, invasion, angiogenesis, and in vivo tumor growth while suppressing apoptosis, partly through sponging miR-145-5p to regulate GFRA1. WTAP-mediated m6A modification increases circ_0032463 stability and expression, supporting a WTAP/circ_0032463/miR-145-5p/GFRA1 mechanism in OS progression. |
| Confidence score: |
0.8386 |