| Expression pattern: |
UP |
| Associated gene: |
EIF4A3, KAT6A, H3K9ac, PCBP2, RHOT1 |
| Associated microRNA: |
- |
| Biological function: |
Circ_0005397 is upregulated in pancreatic cancer, predicts poor prognosis, promotes tumor growth and cell viability, and inhibits erastin-induced ferroptosis; its knockdown sensitizes pancreatic cancer cells to ferroptosis and reduces tumor growth. |
| Molecular mechanism: |
EIF4A3 induces circ_0005397 expression by binding flanking sequences of RHOT1; nuclear circ_0005397 binds KAT6A and promotes KAT6A/H3K9ac-mediated transcriptional activation of PCBP2, thereby inhibiting erastin-induced ferroptosis. |
| Biological pathway or process: |
ferroptosis (inhibits); proliferation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; Transfection; MTT; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Increased circ_0005397 expression was associated with poor prognosis and may serve as a prognostic biomarker and therapeutic target in pancreatic cancer. |
| Description: |
This study identifies hsa_circ_0005397/circRHOT1 as an upregulated nuclear circRNA in pancreatic cancer associated with poor patient survival. EIF4A3 induces circ_0005397 expression, and circ_0005397 binds KAT6A to enhance H3K9ac-mediated PCBP2 transcription, thereby suppressing erastin-induced ferroptosis and supporting tumor progression. Knockdown of circ_0005397 sensitizes pancreatic cancer cells and xenograft tumors to ferroptosis, suggesting therapeutic and prognostic relevance. |
| Confidence score: |
0.8954 |