| Expression pattern: |
UP |
| Associated gene: |
NAMPT |
| Associated microRNA: |
miR-142-5p |
| Biological function: |
promotes chondrocyte proliferation, ECM generation and inhibits apoptosis and inflammation when silenced; overall accelerates OA progression |
| Molecular mechanism: |
ceRNA mechanism; sponges miR-142-5p to regulate NAMPT |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); inflammation (promotes); ceRNA regulation (promotes); mRNA stability (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; MTT; EdU Staining; Annexin V/PI Flow Cytometry; ELISA; Western Blot |
| Clinical significance: |
circ-NT5C2 may serve as a molecular marker for OA diagnosis and a potential therapeutic target |
| Description: |
circ-NT5C2 is upregulated in osteoarthritis and promotes disease progression. Mechanistically, it acts as a ceRNA that sponges miR-142-5p, relieving repression of NAMPT and thereby promoting inflammation, apoptosis, and reduced proliferation/ECM synthesis in IL-1beta-stimulated chondrocytes. Silencing circ-NT5C2 reverses these OA-like phenotypes in vitro. |
| Confidence score: |
0.7244 |