| Expression pattern: |
DS |
| Associated gene: |
IL-8, PD-L1, AGO 2 |
| Associated microRNA: |
miR-146a-5p |
| Biological function: |
Promotes gastric cancer cell migration, invasion and metastasis by increasing IL-8 secretion from GC-MSCs; enhances PD-L1 expression in gastric cancer cells and promotes resistance to cytotoxic CD8 + T cell-killing. |
| Molecular mechanism: |
circ_0073453 acts as a ceRNA sponge for miR-146a-5p, relieving miR-146a-5p-mediated repression of IL-8, thereby increasing IL-8 expression and secretion; secreted IL-8 enhances PD-L1 expression and immune escape. |
| Biological pathway or process: |
ceRNA regulation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); immune regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Transwell Assay; ELISA; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circ_0073453/miR-146a-5p/IL-8 axis was deregulated in gastric cancer tissues and associated with prognosis depending on MSC abundance; hsa_circ_0073453 may be a potential therapeutic target for gastric cancer. |
| Description: |
This study identifies hsa_circ_0073453/circ_0073453 as a GC-MSC-derived circRNA involved in gastric cancer progression. It functions as a sponge for miR-146a-5p to increase IL-8 expression and secretion, thereby promoting gastric cancer cell migration, invasion, metastasis, PD-L1 upregulation, and resistance to CD8 + T cell cytotoxicity. |
| Confidence score: |
0.8625 |