| Expression pattern: |
DN |
| Associated gene: |
MMP16 |
| Associated microRNA: |
miR-23a-3p |
| Biological function: |
Circ_0122396 protects HLECs against H2O2-induced apoptotic and oxidative injury by promoting cell proliferation, inhibiting apoptosis, reducing MDA content, and increasing GSH-PX activity. |
| Molecular mechanism: |
Circ_0122396 acts as a miR-23a-3p sponge to regulate MMP16 expression in H2O2-stimulated HLECs. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (promotes); apoptosis (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot |
| Clinical significance: |
Circ_0122396 may serve as a potentially valuable biomarker and novel therapeutic target for ARC. |
| Description: |
Circ_0122396 is down-regulated in ARC anterior lens capsules. In H2O2-stimulated human lens epithelial cells, circ_0122396 overexpression promotes proliferation and suppresses apoptosis and oxidative injury. Mechanistically, circ_0122396 functions as a miR-23a-3p sponge to regulate MMP16, forming a circ_0122396-miR-23a-3p-MMP16 axis with potential biomarker and therapeutic relevance for ARC. |
| Confidence score: |
0.8107 |