| Expression pattern: |
DN |
| Associated gene: |
KLF9, Ago2 |
| Associated microRNA: |
miR-889 |
| Biological function: |
Inhibits OS cell growth, migration, invasion and EMT, and promotes apoptosis; suppresses in vivo lung metastasis. |
| Molecular mechanism: |
ceRNA mechanism: circ_0078767 sponges miR-889 to relieve repression of KLF9, increasing KLF9 expression. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); apoptosis (promotes); metastasis (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Wound Healing Assay; Transwell Assay; Western Blot; TUNEL; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; H&E Staining; Clinical Sample Validation; Survival Analysis |
| Clinical significance: |
Low circ_0078767 expression was associated with shorter overall survival time of OS patients; low expression correlated with increased T stage. |
| Description: |
circ_0078767 is down-regulated in osteosarcoma and acts as a tumor suppressor. It sponges miR-889 to upregulate KLF9, thereby inhibiting proliferation, migration, invasion and EMT while promoting apoptosis, and it is associated with better overall survival when highly expressed. |
| Confidence score: |
0.8635 |