| Expression pattern: |
UP |
| Associated gene: |
ELK4 |
| Associated microRNA: |
miR-520f-3p |
| Biological function: |
Promotes glioma cell proliferation, invasion, and migration; circNUP98 knockdown decreases cell viability, proliferation, invasion, and migration. |
| Molecular mechanism: |
circNUP98 functions as a ceRNA sponge for miR-520f-3p, relieving miR-520f-3p-mediated repression of ELK4. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot |
| Clinical significance: |
Potential therapeutic target for glioma treatment. |
| Description: |
circNUP98 is upregulated in human glioma cell lines and acts as an oncogenic circRNA. It promotes glioma cell viability, proliferation, invasion, and migration by sponging miR-520f-3p and relieving repression of ELK4. The study proposes circNUP98 as a potential therapeutic target for glioma treatment. |
| Confidence score: |
0.7615 |