| Expression pattern: |
UP |
| Associated gene: |
target mRNAs |
| Associated microRNA: |
miR-515, miR-942, miR-589, miR-136, miR-620, miR-630 |
| Biological function: |
promotes ESCC cell proliferation |
| Molecular mechanism: |
Predicted miRNA sponge (ceRNA-like) via bioinformatics interaction network (not experimentally validated) |
| Biological pathway or process: |
proliferation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; ROC Analysis; Transfection; CCK8; Colony Formation Assay; Bioinformatics Analysis |
| Clinical significance: |
Potential diagnostic biomarker in plasma (AUC 0.6480) and associated with TNM stage |
| Description: |
circ-DLG1 (hsa_circ_0007203), derived from DLG1 exons 13-18, is up-regulated in ESCC tissues, plasma, and ESCC cell lines. circ-DLG1 knockdown suppresses ESCC cell proliferation in vitro, and bioinformatics predicts it may sponge multiple miRNAs and relate to a circRNA-miRNA-mRNA network, suggesting biomarker potential in plasma. |
| Confidence score: |
0.743 |