| Expression pattern: |
DN |
| Associated gene: |
QKI, NOVA1, U1 small nuclear RNA, U1 snRNA, PUF60 |
| Associated microRNA: |
- |
| Biological function: |
circ-UBR5 may serve as an indicator of NSCLC differentiation degree and may function in tumor dedifferentiation by regulating RNA splicing; it has no obvious functional phenotype in NSCLC cells. |
| Molecular mechanism: |
circ-UBR5 binds RNA splicing regulatory factors QKI and NOVA1 and U1 snRNA in the nucleus and may act as a novel snRNA involved in spliceosome-mediated RNA splicing regulation. |
| Biological pathway or process: |
other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Transfection; Bioinformatics Analysis |
| Clinical significance: |
circ-UBR5 might serve as an index of NSCLC differentiation and could be used as an indicator of NSCLC differentiation degree. |
| Description: |
circ-UBR5 is a human exonic circRNA derived from the UBR5 gene and is significantly down-regulated in NSCLC tissues. Its low expression is associated with poor tumor differentiation, suggesting clinical value as an indicator of NSCLC differentiation degree. Mechanistically, circ-UBR5 is mainly nuclear and binds QKI, NOVA1, and U1 snRNA, suggesting a role as a novel snRNA-like circRNA in spliceosome-mediated RNA splicing regulation. |
| Confidence score: |
0.7514 |