| Expression pattern: |
DN |
| Associated gene: |
ERCC6 |
| Associated microRNA: |
miR-125a-5p |
| Biological function: |
Protects against H2O2-induced human lens epithelial cell injury by promoting cell viability and proliferation and inhibiting apoptosis and oxidative stress. |
| Molecular mechanism: |
circSTK39 acts as a miR-125a-5p sponge and regulates ERCC6 expression through the miR-125a-5p/ERCC6 pathway. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Bioinformatics Analysis |
| Clinical significance: |
Potential therapeutic relevance for ARC therapy. |
| Description: |
circSTK39 is downregulated in ARC lens tissues and H2O2-induced SRA01/04 cells. Its overexpression protects human lens epithelial cells from H2O2-induced injury by promoting proliferation and inhibiting apoptosis and oxidative stress, acting through a circSTK39/miR-125a-5p/ERCC6 ceRNA axis. |
| Confidence score: |
0.7507 |