| Expression pattern: |
UN |
| Associated gene: |
SIRT1, NF-kappaB, p65 |
| Associated microRNA: |
- |
| Biological function: |
circPVT1-exo enhances self-renewal and proliferation, suppresses cell senescence, maintains tenogenic differentiation capacity, and weakens osteogenic differentiation in aged L-TSPCs. |
| Molecular mechanism: |
Exosomal circPVT1 increases SIRT1 expression and inhibits NF-kappaB pathway activation, thereby ameliorating aging-impaired TSPC function. |
| Biological pathway or process: |
NF-kappaB (inhibits); proliferation (promotes); stemness (promotes); inflammation (inhibits); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; EdU Staining; Colony Formation Assay; Western Blot; IF (Immunofluorescence) |
| Clinical significance: |
circPVT1-exo has therapeutic potential for age-related diseases. |
| Description: |
This study shows that exosomal circPVT1 from young TSPCs can rescue aging-impaired L-TSPC phenotypes by promoting proliferation, self-renewal, stemness, and tenogenic differentiation while reducing senescence and osteogenic differentiation. Mechanistically, circPVT1-exo acts through upregulation of SIRT1 and suppression of NF-kappaB activation, suggesting a potential exosome-based therapeutic strategy for age-related tendon diseases. |
| Confidence score: |
0.421 |