| Expression pattern: |
UP |
| Associated gene: |
MEF2C, JAGGED1, AGO2 |
| Associated microRNA: |
miR-488-3p |
| Biological function: |
Promotes glioma cell proliferation, migration, invasion, metastasis, EMT-related malignant phenotype, and tumor growth in vivo. |
| Molecular mechanism: |
CircVCAN acts as a ceRNA/molecular sponge for miR-488-3p to regulate MEF2C expression; MEF2C promotes JAGGED1 transcription, forming the circVCAN/miR-488-3p/MEF2C-JAGGED1 axis. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
The circVCAN/miR-488-3p/MEF2C-JAGGED1 axis could serve as a potential target for the management of glioma. |
| Description: |
CircVCAN is up-regulated and predominantly cytoplasmic in glioma tissues and cells. It promotes glioma proliferation, migration, invasion, EMT-related malignant phenotypes, and in vivo tumor growth by sponging miR-488-3p to regulate MEF2C and downstream JAGGED1 transcriptional signaling. The circVCAN/miR-488-3p/MEF2C-JAGGED1 axis is proposed as a potential therapeutic target for glioma. |
| Confidence score: |
0.8682 |