| Expression pattern: |
UP |
| Associated gene: |
ITGA5, HuR / ELAVL1 |
| Associated microRNA: |
miR-326 |
| Biological function: |
Stimulates HCC cell proliferation, migration, invasion and tumor growth; suppresses apoptosis; promotes Warburg effect/aerobic glycolysis. |
| Molecular mechanism: |
circ_0000518 enhances ITGA5 expression through ceRNA sponging of miR-326 and an RBP mechanism involving HuR-mediated stabilization of ITGA5 mRNA, thereby activating the Warburg effect. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (promotes); mRNA stability (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Clinical Sample Validation; Actinomycin D / DRB Stability Assay; RNase R Treatment; FISH / smFISH; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; IHC (Immunohistochemistry) |
| Clinical significance: |
It provides a meaningful diagnostic and therapeutic target for HCC. |
| Description: |
hsa_circ_0000518/circ_0000518 is up-regulated in HCC clinical tissues and cell lines and acts as a pro-oncogenic circRNA. It promotes proliferation, migration, invasion, xenograft tumor growth and Warburg-effect-related glycolysis while inhibiting apoptosis. Mechanistically, it increases ITGA5 via both miR-326 sponging and HuR-mediated ITGA5 mRNA stabilization, suggesting diagnostic and therapeutic relevance in HCC. |
| Confidence score: |
0.8571 |