| Expression pattern: |
DN |
| Associated gene: |
SEMA3A |
| Associated microRNA: |
miR-135b |
| Biological function: |
Overexpression of circZNF609 alleviated atherosclerosis-related injury by reducing lipid accumulation, inflammation, mitochondrial dysfunction, necrosis, apoptosis, and cell death in ox-LDL-stimulated macrophages and AS mice. |
| Molecular mechanism: |
circZNF609 acts as a ceRNA sponge for miR-135b, thereby up-regulating SEMA3A expression and suppressing lipid accumulation, inflammatory response, mitochondrial dysfunction, necrosis, apoptosis, and cell death. |
| Biological pathway or process: |
ceRNA regulation (promotes); lipid metabolism (inhibits); inflammation (inhibits); mitochondrial function (promotes); apoptosis (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; Oil Red O Staining; ELISA; Western Blot; Annexin V/PI Flow Cytometry; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
circZNF609 was decreased in peripheral blood of AS patients and negatively correlated with miR-135b; the authors suggest circZNF609 is likely to become a novel candidate therapy for improving AS progression. |
| Description: |
circZNF609 is down-regulated in AS patients and ox-LDL-stimulated macrophage models. Its overexpression protects against atherosclerosis-associated lipid accumulation, inflammation, mitochondrial dysfunction, necrosis, apoptosis, and cell death by sponging miR-135b and increasing SEMA3A expression. |
| Confidence score: |
0.7552 |