circRNA basic information
circBase ID: hsa_circ_0078607
Name: hsa_circ_SLC22A3
Synonym: -
Host Gene: SLC22A3
Genomic location(hg19): chr6:160819010-160831878:+
Genomic location(hg38): chr6:160397978-160410846:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; para-tumorous (PT) tissues
Cell lines:

HepG2; Bel7404
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: CPSF3, SLC22A3, NCAPG2
Associated microRNA: miR-188, miR-1294
Biological function: Acts as a miRNA sponge/storage component; CPSF3-mediated reduction of this circRNA is linked to disrupted miRNA-mediated gene silencing and increased expression of miRNA target genes in HCC cells.
Molecular mechanism: CPSF3 enhances PAS-dependent pre-mRNA cleavage, shifting processing from circRNA (hsa_circ_0078607) to linear mRNA (SLC22A3), reducing circRNA-associated miRNAs (miR-188/miR-1294) and thereby impairing miRNA-mediated silencing of targets such as NCAPG2.
Biological pathway or process:

ceRNA regulation (other)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; Clinical Sample Validation; RNA Pull-Down; Dot Blot
Clinical significance:

-
Description:

In HCC cells, CPSF3 suppresses hsa_circ_0078607 biogenesis (PAS-dependent shift from circRNA to linear SLC22A3 mRNA). Reduced hsa_circ_0078607 is associated with decreased levels of its bound miRNAs (miR-188/miR-1294) and impaired miRNA-mediated gene silencing (e.g., NCAPG2).
Confidence score:

0.5005
Other information
Title:

CPSF3 Promotes Pre-mRNA Splicing and Prevents CircRNA Cyclization in Hepatocellular Carcinoma.

Journal: Cancers
Published: 2023
PubMed ID: 37627085
Study type:

combined biological and clinical study
Data availability: GSE94508; GSE97332; GSE49515; GSE138734; TCGA
Code availability: -