circRNA basic information
circBase ID: hsa_circ_0072088
Name: hsa_circ_ZFR
Synonym: circZFR
Host Gene: ZFR
Genomic location(hg19): chr5:32379220-32388780:-
Genomic location(hg38): chr5:32379114-32388674:-
Subcellular localization: nucleus and cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; adjacent normal tissues; serum; serum exosomes; tumor tissues; tissue microarray
Cell lines:

HCT116; Colo320; 293T
In vivo animal model:

cell line-derived xenograft; patient-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: BCLAF1, RTKN2, ESRP1, AGO-2
Associated microRNA: miR-3127-5p, miR-1266-5p
Biological function: Promotes CRC cell proliferation, migration, invasion, tumor growth, pulmonary and hepatic metastasis, exosome-mediated oncogenic effects, and suppresses apoptosis.
Molecular mechanism: ESRP1 promotes circZFR biogenesis; circZFR binds BCLAF1 and prevents E3-ligase mediated ubiquitination and degradation; circZFR sponges miR-3127-5p to increase RTKN2 expression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); EMT (promotes); ceRNA regulation (promotes); ubiquitination (inhibits); other pathway/process (promotes)
Detected method:
Q
H
M
Validation methods:

Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Clinical Sample Validation; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA-seq; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Digital PCR
Clinical significance:

CircZFR is a promising biomarker for CRC diagnosis and prognosis; high circZFR levels are associated with advanced stage, lymph node and distant metastases, and shorter overall survival; serum circZFR may serve as a liquid biopsy marker.
Description:

CircZFR is an up-regulated oncogenic circRNA in colorectal cancer tissues and serum exosomes, associated with advanced stage, metastasis, diagnostic value, and poor overall survival. Functionally, it promotes CRC proliferation, migration, invasion, tumor growth and metastasis while inhibiting apoptosis. Mechanistically, ESRP1 enhances circZFR biogenesis, circZFR stabilizes BCLAF1 by preventing ubiquitin-proteasome degradation, and circZFR acts as a sponge for miR-3127-5p to increase RTKN2 expression.
Confidence score:

0.9007
Other information
Title:

CircZFR promotes colorectal cancer progression via stabilizing BCLAF1 and regulating the miR-3127-5p/RTKN2 axis.

Journal: Science China. Life sciences
Published: 2024
PubMed ID: 38805063
Study type:

combined biological and clinical study
Data availability: GSE126094; https://doi.org/10.1007/s11427-023-2514-y
Code availability: -