| Expression pattern: |
UP |
| Associated gene: |
IGF2BP3, GLUT1 mRNA |
| Associated microRNA: |
- |
| Biological function: |
circFOXK2 promotes malignant phenotypes of OSCC cells, including proliferation, migration, glucose uptake, lactate production, and ATP levels. |
| Molecular mechanism: |
m6A-modified circFOXK2 cooperates with IGF2BP3 to enhance GLUT1 mRNA stability in a m6A-dependent manner, thereby accelerating aerobic glycolysis in OSCC cells. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); glycolysis (promotes); mRNA stability (promotes); m6A modification (other); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RNase R Treatment; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); FISH / smFISH; Transfection; CCK8; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circFOXK2 may be a potential therapeutic target for OSCC. |
| Description: |
circFOXK2 (hsa_circ_0000816), a FOXK2-derived m6A-modified circRNA, is up-regulated in OSCC cells. It acts as an oncogenic circRNA by promoting OSCC cell proliferation, migration, and aerobic glycolysis. Mechanistically, circFOXK2 cooperates with the m6A reader/RBP IGF2BP3 to enhance GLUT1 mRNA stability in a m6A-dependent manner, suggesting potential therapeutic relevance in OSCC. |
| Confidence score: |
0.7884 |