| Expression pattern: |
UP |
| Associated gene: |
RUNX2 |
| Associated microRNA: |
miR-217 |
| Biological function: |
Promotes retinal reactive gliosis and glial cell activation and contributes to RGC degeneration; cZRANB1 knockdown facilitates RGC survival and reduces Müller cell proliferation/viability. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-217, relieving miR-217 repression of RUNX2 to regulate Müller cell function. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; IF (Immunofluorescence); In Vivo Animal Model; Flow Cytometry（Non-apoptosis/cycle）; Luciferase Reporter Assay; RNA Pull-Down; Western Blot |
| Clinical significance: |
cZRANB1 was significantly upregulated in the aqueous humor from the patients with primary open-angle glaucoma. |
| Description: |
cZRANB1 is up-regulated in glaucoma-associated retinal neurodegeneration and contributes to reactive gliosis and glial activation. Silencing cZRANB1 reduces Müller cell proliferation/activation and indirectly protects RGCs, mainly via a ceRNA mechanism involving miR-217 and RUNX2. |
| Confidence score: |
0.7659 |