| Expression pattern: |
UP |
| Associated gene: |
CCDC6 |
| Associated microRNA: |
hsa-miR-149-5p |
| Biological function: |
promotes GC cell proliferation and growth; promotes lymphatic metastasis/lymph node metastasis |
| Molecular mechanism: |
ceRNA mechanism: circDNA2 sponges miR-149-5p to relieve repression of CCDC6, thereby increasing CCDC6 expression |
| Biological pathway or process: |
proliferation (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Clinical Sample Validation; ROC Analysis |
| Clinical significance: |
High circDNA2 expression is associated with lymphatic invasion/lymph node metastasis; circDNA2 combined with miR-149-5p may help predict GC occurrence (AUC 0.7301); circDNA2 expression was not significantly correlated with OS or survival after chemotherapy |
| Description: |
circDNA2 is up-regulated in gastric cancer and mainly localized in the cytoplasm. It promotes GC cell proliferation and lymphatic metastasis by sponging miR-149-5p, thereby increasing CCDC6 expression. |
| Confidence score: |
0.8588 |