| Expression pattern: |
UP |
| Associated gene: |
polypeptide N-acetylgalactosaminyltransferase 3 (GALNT3), Ago2 |
| Associated microRNA: |
miR-545-5p |
| Biological function: |
Promotes CRC cell proliferation, migration, invasion, and tumor growth; inhibits apoptosis. |
| Molecular mechanism: |
circ-RAPGEF5 functions as a miR-545-5p sponge and upregulates GALNT3 expression through the miR-545-5p/GALNT3 axis. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circ-RAPGEF5 can be used as a biomarker for the diagnosis and prognosis of CRC and may provide potential therapeutic targets. |
| Description: |
circ-RAPGEF5 is a human circRNA derived from RAPGEF5 that is upregulated in CRC tissues and cells. It promotes malignant CRC phenotypes by sponging miR-545-5p and relieving miR-545-5p-mediated repression of GALNT3, thereby enhancing proliferation, migration, invasion, and in vivo tumor growth while suppressing apoptosis. The study suggests circ-RAPGEF5 as a potential biomarker and therapeutic target in CRC. |
| Confidence score: |
0.8635 |