| Expression pattern: |
UP |
| Associated gene: |
KLF4 |
| Associated microRNA: |
miR-7-5p |
| Biological function: |
Circ-PRMT5 stimulates proliferative ability and malignant development in Wilms' tumor cells; knockdown suppresses cell viability, colony formation and EdU-positive rate. |
| Molecular mechanism: |
Circ-PRMT5 activates the miR-7-5p/KLF4 axis, with miR-7-5p binding circ-PRMT5 and KLF4 acting as a downstream gene of miR-7-5p. |
| Biological pathway or process: |
proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
High circ-PRMT5 levels are associated with advanced tumor staging in patients with Wilms' tumor and circ-PRMT5 may serve as a potential biomarker for diagnosis, prognosis and therapeutic response. |
| Description: |
Circ-PRMT5 is upregulated in Wilms' tumor tissues and cells, and higher expression is associated with advanced tumor staging. Functionally, circ-PRMT5 promotes Wilms' tumor cell proliferation, at least partly through regulation of the miR-7-5p/KLF4 axis. |
| Confidence score: |
0.6521 |