| Expression pattern: |
UP |
| Associated gene: |
TIMELESS, METTL3, YTHDC1, Ago2, p-ATR, p-CHK1, RAD51, gamma-H2AX |
| Associated microRNA: |
miR-143-3p |
| Biological function: |
Promotes OC cell proliferation, clone formation, migration and tumor growth; inhibits DNA damage and apoptosis. |
| Molecular mechanism: |
METTL3-mediated m6A modification promotes hsa_circ_0061179 circularization and stability, and YTHDC1 promotes its cytoplasmic transfer; cytoplasmic hsa_circ_0061179 acts as a ceRNA sponge for miR-143-3p to upregulate TIMELESS and activate ATR/CHK1 signaling, thereby inhibiting DNA damage and apoptosis. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); ceRNA regulation (other); m6A modification (other); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; MeRIP / MeRIP-seq; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; IF (Immunofluorescence); IHC (Immunohistochemistry); Western Blot; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High hsa_circ_0061179 expression was associated with inferior survival, advanced FIGO stage, and positive lymph node metastasis in OC patients; the hsa_circ_0061179/miR-143-3p/TIMELESS axis suggests therapeutic target potential. |
| Description: |
hsa_circ_0061179/circZBTB46 is up-regulated in ovarian cancer and high expression is associated with poorer survival and more advanced clinicopathological features. Mechanistically, METTL3-mediated m6A modification enhances hsa_circ_0061179 expression and stability, YTHDC1 promotes its cytoplasmic export, and cytoplasmic hsa_circ_0061179 sponges miR-143-3p to increase TIMELESS and activate ATR/CHK1-related DNA damage repair signaling, thereby promoting proliferation, migration and tumor growth while inhibiting apoptosis and DNA damage. |
| Confidence score: |
0.8933 |