| Expression pattern: |
UP |
| Associated gene: |
GLUL, Ago2 |
| Associated microRNA: |
miR-4673 |
| Biological function: |
Promotes NSCLC cell proliferation, clone formation, cell cycle progression, migration, invasion, tumor growth, and glutamine-related metabolic activity; inhibits apoptosis signaling. |
| Molecular mechanism: |
Circ_0006949 acts as a cytoplasmic ceRNA sponge for miR-4673, preventing miR-4673-mediated repression of GLUL, thereby increasing GLUL expression and catalytic activity, regulating glutamine metabolism and suppressing apoptosis signaling. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glutaminolysis (other); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot |
| Clinical significance: |
Sputum circ_0006949 is a non-invasive biomarker for early diagnosis and therapeutic monitoring of NSCLC; it has higher diagnostic value than conventional serum markers and decreases after surgery. |
| Description: |
Circ_0006949 is up-regulated in sputum and tumor tissues from NSCLC patients and serves as a potential non-invasive biomarker for early diagnosis and therapeutic monitoring. Functionally, cytoplasmic circ_0006949 promotes NSCLC proliferation, colony formation, migration, invasion and xenograft tumor growth, while suppressing apoptosis signaling. Mechanistically, it acts as a ceRNA for miR-4673 to upregulate GLUL, enhancing GLUL catalytic activity and glutamine-related metabolism. |
| Confidence score: |
0.8457 |