| Expression pattern: |
DN |
| Associated gene: |
TET2 |
| Associated microRNA: |
miR-589-5p |
| Biological function: |
circ_0003423 overexpression alleviated ox-LDL-induced HBMEC-IM cell injury by promoting cell viability and tube formation while reducing apoptosis, oxidative stress, inflammation and ER stress. |
| Molecular mechanism: |
circ_0003423 acts as a sponge for miR-589-5p and upregulates TET2, thereby alleviating ox-LDL-induced HBMEC-IM cell injury. |
| Biological pathway or process: |
ceRNA regulation (other); apoptosis (inhibits); angiogenesis (promotes); inflammation (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Tube Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0003423 might be a new potential target for treating cerebrovascular diseases. |
| Description: |
circ_0003423 is down-regulated in ox-LDL-induced human BMEC injury models. Its overexpression protects HBMEC-IM cells by enhancing viability and angiogenesis and suppressing apoptosis, oxidative stress, inflammation and ER stress through the miR-589-5p/TET2 axis. |
| Confidence score: |
0.7992 |