| Expression pattern: |
DN |
| Associated gene: |
ELAVL1, CCND2 |
| Associated microRNA: |
- |
| Biological function: |
Overexpression of circ_0002331 promotes proliferation, migration, invasion and angiogenesis, and inhibits apoptosis, autophagy and inflammation in ox-LDL-induced HUVECs, thereby protecting against endothelial cell dysfunction in atherosclerosis. |
| Molecular mechanism: |
circ_0002331 binds the RNA-binding protein ELAVL1 and promotes CCND2 mRNA stability, leading to increased CCND2 expression and protection of HUVECs from ox-LDL-induced dysfunction. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); autophagy (inhibits); inflammation (inhibits); mRNA stability (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; ELISA; Western Blot; ROC Analysis; Bioinformatics Analysis; Nuclear-Cytoplasmic Fractionation |
| Clinical significance: |
circ_0002331 might be a diagnostic biomarker for AS patients; ROC assay showed an AUC of 0.8867. |
| Description: |
circ_0002331 is downregulated in AS patient serum and ox-LDL-induced HUVECs. It protects endothelial cells from ox-LDL-induced dysfunction by interacting with ELAVL1 to stabilize CCND2 mRNA, promoting proliferation, migration, invasion and angiogenesis while inhibiting apoptosis, autophagy and inflammation. Its serum level also shows potential diagnostic value for AS. |
| Confidence score: |
0.8442 |