| Expression pattern: |
UN |
| Associated gene: |
FLI1 promoter, TET1, DNMT1 |
| Associated microRNA: |
- |
| Biological function: |
Promotes invasion/metastasis of breast cancer cells and activates FLI1 transcription via epigenetic regulation. |
| Molecular mechanism: |
FECR1 binds the FLI1 promoter in cis and recruits TET1 to demethylate CpG islands; FECR1 also binds the DNMT1 promoter and downregulates DNMT1, contributing to FLI1 promoter hypomethylation and increased FLI1 expression. |
| Biological pathway or process: |
invasion (promotes); metastasis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; RNA Pull-Down; RIP (RNA Immunoprecipitation); ChIP / ChIP-seq; Transfection; Transwell Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
FECR1 (an exonic circRNA from FLI1) promotes invasion/metastasis in breast cancer cells. Mechanistically, it binds the FLI1 promoter and recruits TET1 to induce CpG demethylation, while also repressing DNMT1, resulting in increased FLI1 expression via an epigenetic positive-feedback loop. |
| Confidence score: |
0.8191 |