| Expression pattern: |
DN |
| Associated gene: |
- |
| Associated microRNA: |
- |
| Biological function: |
Potential hepatocellular carcinoma biomarker used as a model target for ultrasensitive and specific circRNA detection; no disease-driving biological function was experimentally tested. |
| Molecular mechanism: |
RETA-CRISPR detection mechanism based on RT-RCA amplification of BSJ-containing repeat units and Cas12a/crRNA recognition to trigger collateral cleavage; no disease-related molecular mechanism was investigated. |
| Biological pathway or process: |
not specified |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Sanger Sequencing; RT-qPCR |
| Clinical significance: |
Potential hepatocellular carcinoma biomarker; method holds promise for diagnosis of circRNA-related diseases, but clinical samples were not tested. |
| Description: |
This study used hsa_circ_0001445 (circRNA1445) as a model potential HCC biomarker to establish and validate the RETA-CRISPR detection platform. circRNA1445 expression was detected at lower levels in the hepatocellular carcinoma cell line HepG2 than in the normal hepatic cell line LO2, with agreement between RETA-CRISPR and RT-qPCR. No disease-related functional mechanism or regulatory axis was experimentally investigated. |
| Confidence score: |
0.385 |