circRNA basic information
circBase ID: hsa_circ_0000517
Name: hsa_circ_RPPH1
Synonym: hsa_circRPPH1_015
Host Gene: RPPH1
Genomic location(hg19): chr14:20811404-20811492:-
Genomic location(hg38): chr14:20343245-20343333:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

breast cancer tissues; adjacent normal tissues
Cell lines:

MCF-7; MDA-MB-435; MDA-MB-231; BCAP; HBL-100
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ELK1, Ago2
Associated microRNA: miR-326
Biological function: promotes proliferation; promotes invasion; promotes cell cycle progression; promotes tumor growth and metastasis in vivo
Molecular mechanism: ceRNA-like mechanism: hsa_circRPPH1_015 sponges miR-326, relieving miR-326-mediated inhibition of ELK1; interacts with Ago2
Biological pathway or process:

proliferation (promotes); invasion (promotes); cell cycle (promotes); metastasis (promotes); EMT (other); angiogenesis (promotes); ceRNA regulation (promotes)
Detected method:
Q
B
M
Validation methods:

Microarray; Northern Blot; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Western Blot; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry)
Clinical significance:

High hsa_circRPPH1_015 expression is associated with worse survival and adverse clinicopathological features; an unfavorable prognostic factor in BC.
Description:

In breast cancer, hsa_circRPPH1_015 is up-regulated and functions as an oncogenic circRNA that promotes proliferation, invasion, cell-cycle progression, and xenograft tumor growth/metastasis. Mechanistically, it binds (sponges) miR-326 in an Ago2-associated manner, relieving miR-326-mediated repression of ELK1, thereby enhancing ELK1-driven malignant phenotypes. Clinically, high hsa_circRPPH1_015 expression is associated with worse patient survival.
Confidence score:

0.8648
Other information
Title:

A Novel Circular RNA hsa_circRPPH1_015 Exerts an Oncogenic Role in Breast Cancer by Impairing miRNA-326-Mediated ELK1 Inhibition.

Journal: Frontiers in oncology
Published: 2020
PubMed ID: 32670874
Study type:

combined biological and clinical study
Data availability: GSE101123; GSE35412
Code availability: -