| Expression pattern: |
UP |
| Associated gene: |
CELF2, GPX4, SLC7A11, ACSL4 |
| Associated microRNA: |
miR-208a-3p |
| Biological function: |
circUQCRC2 promotes H/R-induced tubular epithelial cell apoptosis, inflammation, and ferroptosis; knockdown of circUQCRC2 increases cell viability and inhibits apoptosis, proinflammatory cytokine production, Fe2+/ROS accumulation, and ferroptosis marker changes in H/R-exposed HK-2 cells. |
| Molecular mechanism: |
circUQCRC2 acts as a miR-208a-3p-interacting circRNA and negatively regulates miR-208a-3p, thereby modulating CELF2 expression and CELF2-mediated apoptosis, inflammation, and ferroptosis in AKI models. |
| Biological pathway or process: |
apoptosis (promotes); inflammation (promotes); ferroptosis (promotes); proliferation (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
circUQCRC2 is up-regulated in H/R-stimulated HK-2 cells and I/R-injured mouse kidneys. It promotes AKI-related tubular epithelial cell injury by negatively regulating miR-208a-3p and increasing CELF2-mediated apoptosis, inflammation, and ferroptosis; circUQCRC2 knockdown protects H/R-injured HK-2 cells. |
| Confidence score: |
0.6435 |