| Expression pattern: |
UP |
| Associated gene: |
JMJD1C, SOX4, AGO2 |
| Associated microRNA: |
miR-182-5p |
| Biological function: |
Promotes breast cancer cell proliferation, invasion, migration, EMT, tumor growth and metastasis; inhibits apoptosis. |
| Molecular mechanism: |
circ_JMJD1C acts as a ceRNA sponge for miR-182-5p to upregulate JMJD1C, and the circ-JMJD1C/JMJD1C axis epigenetically activates SOX4 to promote EMT and breast cancer progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); apoptosis (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Western Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; TUNEL; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
High expression of circ_JMJD1C predicted poor clinical outcomes and may be a promising therapeutic target for breast cancer. |
| Description: |
circ_JMJD1C is up-regulated in breast cancer tissues and cells and functions as an oncogenic circRNA. It promotes proliferation, migration, invasion, EMT, tumor growth and metastasis while suppressing apoptosis, mainly through sponging miR-182-5p to increase JMJD1C and epigenetically activate SOX4. High circ_JMJD1C expression is reported to predict poor clinical outcomes, suggesting therapeutic potential for targeting circ_JMJD1C in breast cancer. |
| Confidence score: |
0.8459 |