circRNA basic information
circBase ID: -
Name: mmu_circ_CDR1as
Synonym: CDR1as
Host Gene: CDR1as
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0043797
MONDO name: spinal cord injury
Disease details: spinal cord injury
Disease DO ID:
 -
Disease MeSH ID:
 D013119
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Mouse
Species details: Mus musculus
Tissue specimen:

spinal cord tissue; lesion epicenter; spinal fibroblasts
Cell lines:

293 T; BV2
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
DN
Associated gene: TGF-betaR2, Smad2, Smad3, phosphorylated-Smad2, phosphorylated-Smad3
Associated microRNA: miR-7a-5p
Biological function: CDR1as affects scar formation, inflammation, apoptosis, nerve regeneration, glial and nerve function, NSC differentiation, microglia polarization, and functional repair after SCI; inhibition of CDR1as reduces fibrosis-associated proteins and improves post-injury recovery.
Molecular mechanism: CDR1as acts through a CDR1as/miR-7a-5p/TGF-betaR2 regulatory axis to regulate fibrosis and Smad pathway activation; siCDR1as inhibits fibrosis-associated proteins and Smad2/3 phosphorylation, and miR-7a-5p inhibitor antagonizes siCDR1as effects.
Biological pathway or process:

TGF-beta/SMAD (promotes); fibrosis (promotes); apoptosis (promotes); inflammation (promotes); immune regulation (promotes); ceRNA regulation (other); other pathway/process (other)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; RNA-seq; Transfection; Luciferase Reporter Assay; IF (Immunofluorescence); IHC (Immunohistochemistry); TUNEL; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis
Clinical significance:

-
Description:

This study identifies mouse CDR1as as a down-regulated circRNA in the lesion epicenter after spinal cord injury and validates its expression by qRT-PCR and junction sequencing. Functionally, inhibiting CDR1as reduces spinal fibrotic scarring, inflammation, apoptosis, glial activation, and Smad2/3 phosphorylation while improving neural repair and locomotor recovery. Mechanistically, CDR1as is proposed to act through the miR-7a-5p/TGF-betaR2 axis to regulate TGF-beta/Smad signaling in spinal fibrosis.
Confidence score:

0.7795
Other information
Title:

CircRNA CDR1as affects functional repair after spinal cord injury and regulates fibrosis through the SMAD pathway.

Journal: Pharmacological research
Published: 2024
PubMed ID: 38649124
Study type:

biological research
Data availability: Data will be made available on request; Supplemental files 1; Supplemental files 2; Supplemental files 3; Supplemental files 4-5; Supplementary data associated with this article can be found in the online version at doi:10.1016/j.phrs.2024.107189.
Code availability: Not applicable.