| Expression pattern: |
DN |
| Associated gene: |
FNDC5 |
| Associated microRNA: |
miR-770-5p |
| Biological function: |
hsa_circ_0006260 overexpression attenuated HG-mediated suppression of HTR-8/SVneo cell viability and migration, reduced inflammatory response, and weakened apoptosis; hsa_circ_0006260 downregulation promoted HG-induced trophoblast cell dysfunction. |
| Molecular mechanism: |
hsa_circ_0006260 acts as a miR-770-5p decoy to upregulate FNDC5 expression, thereby reducing HG-induced HTR-8/SVneo cell dysfunction. |
| Biological pathway or process: |
ceRNA regulation (promotes); migration (promotes); apoptosis (inhibits); inflammation (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; ELISA; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0006260 was a probable target for GDM treatment |
| Description: |
hsa_circ_0006260 is down-regulated in placentas from GDM patients and in HG-stimulated HTR-8/SVneo trophoblast cells. It primarily localizes to the cytoplasm and functions as a miR-770-5p decoy, thereby upregulating FNDC5 and protecting trophoblast cells from HG-induced dysfunction, including reduced viability and migration, increased inflammatory cytokine response, and apoptosis. The study proposes hsa_circ_0006260 as a potential therapeutic target for GDM. |
| Confidence score: |
0.7977 |