circRNA basic information
circBase ID: hsa_circ_0006332
Name: hsa_circ_MYBL2
Synonym: -
Host Gene: MYBL2
Genomic location(hg19): chr20:42331129-42333998:+
Genomic location(hg38): chr20:43702489-43705358:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

colorectal cancer primary specimens; matched adjacent normal tissues; colorectal cancer tissues; lung tissues
Cell lines:

HEK293T; NCM460; HCT116; SW480; LoVo; SW620; HT29; RKO; SW48; DLD-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: p185, UCHL3, PHGDH
Associated microRNA: -
Biological function: circMYBL2 encodes p185, which suppresses colorectal cancer cell growth, proliferation, invasion, migration, metastasis and aggressiveness in vitro and in vivo.
Molecular mechanism: circMYBL2 encodes a 185-aa protein p185; p185 competitively binds the C1 domain of UCHL3, counteracts UCHL3-mediated PHGDH deubiquitination, promotes PHGDH degradation, and reduces serine and glycine biosynthesis.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); ubiquitination (promotes); other pathway/process (inhibits)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; Western Blot; Co-IP; IF (Immunofluorescence); Bioinformatics Analysis
Clinical significance:

low p185 expression was significantly related to tumor invasion depth and lymph node metastasis in patients with colorectal cancer
Description:

circMYBL2 is downregulated in colorectal cancer tissues and cell lines and encodes the 185-aa protein p185. p185 acts as a tumor suppressor by competitively binding UCHL3, reducing UCHL3-mediated deubiquitination of PHGDH, promoting PHGDH degradation, and lowering serine and glycine biosynthesis, thereby inhibiting colorectal cancer growth, invasion and metastasis.
Confidence score:

0.8547
Other information
Title:

The circMYBL2-Encoded p185 Protein Suppresses Colorectal Cancer Progression by Inhibiting Serine Biosynthesis.

Journal: Cancer research
Published: 2024
PubMed ID: 38635900
Study type:

combined biological and clinical study
Data availability: GSE109676; GSE126094; GSE138589; GSE142837; GSE159669; GSE172229; PXD051064
Code availability: -