| Expression pattern: |
DN |
| Associated gene: |
SMAD4 |
| Associated microRNA: |
miR-145-5p , miR-7-5p |
| Biological function: |
Potential novel biomarker for AchR + MG; interrelated with the development of AchR + MG and identified as a hub circRNA with high degree in the DECM network. |
| Molecular mechanism: |
Included in circRNA-miRNA-mRNA ceRNA network analysis and associated with MG-related immune pathways through predicted/integrated miRNA-mRNA interactions; no direct experimental validation of a specific circNUP214 axis was reported in MG. |
| Biological pathway or process: |
ceRNA regulation (other); immune regulation (other); MAPK (other); Wnt/beta-catenin (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Potential novel biomarker for AchR + MG; diagnostic ROC AUC = 0.8288. |
| Description: |
hsa_circ_0089153/circNUP214 is down-regulated in PBMCs from MG patients and was validated by RT-qPCR. It was identified as a hub circRNA in the ceRNA network and proposed as a potential diagnostic biomarker for AchR-positive MG, but its specific MG mechanism was not directly validated. |
| Confidence score: |
0.6556 |