circRNA basic information
circBase ID: hsa_circ_0005015
Name: hsa_circ_HAS2
Synonym: Circ_0005015
Host Gene: HAS2
Genomic location(hg19): chr8:122640953-122641580:-
Genomic location(hg38): chr8:121628713-121629340:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0018177
MONDO name: glioblastoma
Disease details: glioblastoma
Disease DO ID:
3068
Disease MeSH ID:
D005909
Disease NCIt ID:
C3058
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue specimens; normal brain tissues; GBM tissues; glioma tissues

Cell lines:

NHA; A172; T98G; U251; LN229

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: BACH1
Associated microRNA: miR-382-5p
Biological function: Promotes GBM cell proliferation, migration, invasion, glycolysis and tumor growth, and suppresses apoptosis.
Molecular mechanism: circ_0005015 acts as a miRNA sponge for miR-382-5p to up-regulate BACH1 expression in GBM.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); glycolysis (promotes); apoptosis (inhibits); ceRNA regulation (other)

Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)

Clinical significance:

circ_0005015 may provide a potential target or marker for GBM diagnosis, treatment, and prognosis.

Description:

circ_0005015 is up-regulated in GBM tissues and cells and functions as an oncogenic circRNA. It promotes GBM proliferation, migration, invasion, glycolysis, and xenograft tumor growth while inhibiting apoptosis through the circ_0005015/miR-382-5p/BACH1 axis. The study suggests circ_0005015 may be a potential therapeutic target or biomarker for GBM.

Confidence score:

0.8443

Other information
Title:

Circ_0005015 upregulates BACH1 to promote aggressive behaviors in glioblastoma by sponging microRNA-382-5p.

Journal: Naunyn-Schmiedeberg's archives of pharmacology
Published: 2024
PubMed ID: 38032493
Study type:

combined biological and clinical study

Data availability: GSE109569; available from the corresponding author on reasonable request; supplementary material available at https:// doi. org/ 10. 1007/ s00210-023-02868-w
Code availability: -