| Expression pattern: |
UP |
| Associated gene: |
ZEB1, EIF4A3, AGTPBP1, E-cadherin, N-cadherin |
| Associated microRNA: |
miR-205-5p |
| Biological function: |
Promotes cadmium-induced EMT during malignant transformation; overexpression enhances EMT marker changes, while knockdown reverses EMT marker changes. |
| Molecular mechanism: |
EIF4A3 binds the parental gene AGTPBP1 to promote circ_000999 expression; cytoplasmic circ_000999 acts as a ceRNA that binds miR-205-5p, inhibits miR-205-5p activity, upregulates ZEB1, and promotes EMT. |
| Biological pathway or process: |
EMT (promotes); ceRNA regulation (promotes); fibrosis (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; IF (Immunofluorescence); Western Blot; Clinical Sample Validation; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circ_000999 may be a potential molecular target and diagnostic marker for LC; its expression is increased in human LC tissues and correlated with EMT indicators. |
| Description: |
circ_000999 is up-regulated during cadmium-induced malignant transformation and in human LC tissues. EIF4A3 promotes circ_000999 expression via the parental gene AGTPBP1, and cytoplasmic circ_000999 promotes EMT by sponging miR-205-5p and increasing ZEB1 expression. The study suggests circ_000999 as a potential diagnostic marker and therapeutic target for LC related to EMT. |
| Confidence score: |
0.8356 |