| Expression pattern: |
UP |
| Associated gene: |
IGF2BP3, CDK2 mRNA |
| Associated microRNA: |
- |
| Biological function: |
Promotes BCa cell proliferation, colony formation, cell cycle progression, and tumor growth in vivo. |
| Molecular mechanism: |
hsa_circ_0005320 interacts with and upregulates IGF2BP3, and IGF2BP3 binds to and enhances CDK2 mRNA stability, thereby affecting cell proliferation and the cell cycle. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); mRNA stability (promotes); m6A modification (other) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; RNA-seq; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Actinomycin D / DRB Stability Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Western Blot; In Vivo Animal Model; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
High expression is associated with tumor differentiation and depth of invasion in BCa patients; hsa_circ_0005320 has potential as a diagnostic marker and therapeutic target for BCa, with ROC AUC of 0.90. |
| Description: |
hsa_circ_0005320 is upregulated in bladder cancer tissues and is associated with unfavorable clinicopathologic characteristics, including tumor differentiation and invasion depth. Functionally, it promotes BCa cell proliferation, colony formation, cell cycle progression, and in vivo tumor growth. Mechanistically, it binds and upregulates IGF2BP3, which enhances CDK2 mRNA stability, forming a pro-oncogenic hsa_circ_0005320/IGF2BP3/CDK2 axis. |
| Confidence score: |
0.8399 |