| Expression pattern: |
DN |
| Associated gene: |
RUNX3, AGO2, p53, p21 |
| Associated microRNA: |
miR-761 |
| Biological function: |
suppresses HCC cell proliferation; mediates G1/S cell cycle arrest; induces cellular senescence; inhibits tumor growth in vivo |
| Molecular mechanism: |
acts as a competing endogenous RNA (miRNA sponge) for miR-761 to relieve inhibition on RUNX3, activating downstream p53/p21 signaling |
| Biological pathway or process: |
proliferation (inhibits); cell cycle (inhibits); apoptosis (other); ceRNA regulation (promotes); p53 signaling (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Western Blot; IHC (Immunohistochemistry); RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; In Vivo Animal Model; H&E Staining; Survival Analysis; Clinical Sample Validation; Cohort Study; Bioinformatics Analysis |
| Clinical significance: |
Low expression level of circLARP4 independently predicted poor survival outcome; decreased circLARP4 level was an independent prognostic factor for poor overall survival and recurrence-free survival in HCC |
| Description: |
circLARP4 is downregulated in HCC and functions as a tumor suppressor by inducing cellular senescence and G1/S arrest and inhibiting proliferation. Mechanistically, it sponges miR-761 to upregulate RUNX3 and activate the p53/p21 senescence program. Low circLARP4 expression is associated with worse survival outcomes. |
| Confidence score: |
0.8497 |