circRNA basic information
circBase ID: hsa_circ_0027791
Name: hsa_circ_NTN4
Synonym: circ_0027791
Host Gene: -
Genomic location(hg19): chr12:96076482-96181246:-
Genomic location(hg38): chr12:95682706-95787468:-
Subcellular localization: unclear
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; matched normal samples; HCC tumor tissues; adjacent normal tissues; peripheral blood
Cell lines:

MHCC97; Hep3B; Huh-7; THLE-2; THP-1; HEK-293 T; PBMCs; CIK cells
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PDL1, METTL3
Associated microRNA: miR-496
Biological function: circ_0027791 promotes HCC cell viability, proliferation, invasion, sphere formation, M2 macrophage polarization, immune evasion, and tumor growth, while inhibiting apoptosis and antitumor immune responses.
Molecular mechanism: METTL3-mediated m6A methylation stabilizes circ_0027791; circ_0027791 acts as a miR-496 sponge to upregulate PDL1, thereby promoting malignant progression and immune evasion in HCC.
Biological pathway or process:

proliferation (promotes); invasion (promotes); stemness (promotes); apoptosis (inhibits); immune regulation (promotes); macrophage polarization (promotes); ceRNA regulation (other); m6A modification (other); other pathway/process (promotes)
Detected method:
Q
M
Validation methods:

RNase R Treatment; RT-qPCR; Microarray; MTT; EdU Staining; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; Transwell Assay; ELISA; Western Blot; IHC (Immunohistochemistry); Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; MeRIP / MeRIP-seq; Transfection; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis; Nuclear-Cytoplasmic Fractionation
Clinical significance:

High circ_0027791 expression is associated with lower overall survival and may provide a new prognostic and therapeutic marker for HCC.
Description:

circ_0027791 is upregulated in HCC tissues and cells, and high expression is associated with poorer overall survival. Functionally, it promotes HCC proliferation, invasion, sphere formation, tumor growth, M2 macrophage polarization, and immune evasion. Mechanistically, METTL3-mediated m6A modification stabilizes circ_0027791, which sponges miR-496 to increase PDL1 expression.
Confidence score:

0.8611
Other information
Title:

Circ_0027791 contributes to the growth and immune evasion of hepatocellular carcinoma via the miR-496/programmed cell death ligand 1 axis in an m6A-dependent manner.

Journal: Environmental toxicology
Published: 2024
PubMed ID: 38546290
Study type:

combined biological and clinical study
Data availability: GSE166678; TCGA-LIHC; The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -