| Expression pattern: |
UP |
| Associated gene: |
BECN1, Ago2 |
| Associated microRNA: |
miR-326 |
| Biological function: |
promotes migration, invasion, proliferation, and angiogenesis; inhibits apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-326 to relieve miR-326-mediated inhibition of BECN1, thereby upregulating BECN1. |
| Biological pathway or process: |
ceRNA regulation (promotes); migration (promotes); invasion (promotes); proliferation (promotes); angiogenesis (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Wound Healing Assay; Transwell Assay; Colony Formation Assay; Tube Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0020850 level was associated with low overall survival; circ_0020850 expression was closely associated with TNM stages, tumor size, and invasion depth. |
| Description: |
circ_0020850 is upregulated in lung adenocarcinoma and acts as an oncogenic circRNA. It promotes malignant phenotypes and tumor growth by sponging miR-326 to derepress BECN1 (circ_0020850/miR-326/BECN1 ceRNA axis). High circ_0020850 expression is associated with worse overall survival and more advanced clinicopathological features. |
| Confidence score: |
0.835 |