| Expression pattern: |
UP |
| Associated gene: |
WNT2B |
| Associated microRNA: |
miR-346 |
| Biological function: |
hsa_circ_0008360 promotes high glucose-induced HK2 cell damage by reducing cell viability and promoting apoptosis, inflammatory response, and fibrotic protein synthesis; its knockdown attenuates these HG-induced effects. |
| Molecular mechanism: |
ceRNA mechanism: hsa_circ_0008360 acts as a miR-346 sponge and regulates WNT2B expression through the miR-346/WNT2B axis. |
| Biological pathway or process: |
ceRNA regulation (other); apoptosis (promotes); inflammation (promotes); fibrosis (promotes); proliferation (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0008360 is proposed as a promising therapeutic target for diabetic nephropathy. |
| Description: |
hsa_circ_0008360 is up-regulated in serum from diabetic nephropathy patients and in high glucose-treated HK2 cells. It promotes HG-induced renal tubular epithelial cell injury by sponging miR-346 and increasing WNT2B expression, thereby enhancing apoptosis, inflammation, and fibrosis while reducing cell viability. The study suggests hsa_circ_0008360 as a potential therapeutic target for DN. |
| Confidence score: |
0.8111 |